DEPC vs. DMPC
nicholas_theodorakis at urmc.rochester.edu
Tue Aug 10 08:55:38 EST 1999
In article <rhubner-0908992343220001 at macgens8.uia.ac.be>,
rhubner at gins.uia.ac.be (Roland Hübner) wrote:
> > >Does anybody any simple chemical(s) other than DEPC which can
> > >neutralize RNase? Jeff
> > I'm sure someone in this newsgroup within the last year mentioned
> > dimethylpyrocarbonate.
> > ... was obviously not as toxic as DEPC but still killed
> > RNases.
> > Duncan
> such claims intrigued me a lot two years ago and I asked a chemist about
> what he thinks -> DMPC is WAY more UNstable than DEPC in acqueous
> Therefore, I switched back to DEPC, when no chaotropic
> salts/detergents/etc. present; for the rest I just use good quality (HPLC)
> water and clean material...
> BTW, there was a method described (Biochemica by BM) to treat in situ
> sections with DEPC to inactivate the RNases... anybody compared this with
> DMPC yet?
> Of course (!), I wonder what would happen to the mRNA on those slides...
Since D(E/M)PC modifies nucleic acids as well, I would think that the
greater instability of DMPC in water would be an advantage, since there
would be a lesser chance that some would remain in solution when it came
in contact with the RNA.
However, I agree that if you have good water and good reagents, you
don't need it at all. I haven't used DEPC in years, and my RNA hasn't
| Nick Theodorakis |
| nicholas_theodorakis at urmc.rochester.edu |
| (previously theodorn at gusun.georgetown.edu) |
Sent via Deja.com http://www.deja.com/
Share what you know. Learn what you don't.
More information about the Methods