fast DNA from mammalian cells

Mircea Podar mpodar at ems.salk.edu
Tue Aug 10 13:26:43 EST 1999


Hello!

Does anyone have a fast protocol to get DNA for PCR from a small number of
cells in culture? I presume just throwing the cells in the PCR mix would
work (I've done it with yeast and coli), but I would like a cleaner way,
and that way the mini DNA sample can be used repeatedly over several days.


Thanks,

Mircea Podar



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