inverted ECL signals?

Soo Jung Yoon j2w19may at
Thu Aug 12 04:53:07 EST 1999

Hello everyone?

Is there anybody who have ever experienced 'inverted signals'  (i.e.
black bands or dots appear white(transparent), and the background is
unusually dark on the film) in ECL detection? I often have these
'inverted signals' in Western or Dot blots, but have no idea about the
cause of it.

What would be the cause of this queer phenomenon? How can I prevent

I would appreciate any help.
Thanks in advance.

Best Regards,
Soo Jung Yoon

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