Problems with ODing Maxiprep

Rick00100 rick00100 at aol.com
Wed Aug 11 17:04:55 EST 1999


Hi all,

Recently I've been having problem ODing my Qiagen maxi-prep DNA.  After
resuspend my DNA pellet in TE (and usually heat at 37C for a few minutes), I
would get a reading of ng/ul DNA.  When I run the same amount on a gel I would
see a boat load (>2ug/ul) of plasmids (relative to a Lambda Hind3 ladder).  Can
someone give me some insights or comments please?

Ricky



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