TBE precipitation

Thu Aug 12 12:00:39 EST 1999

I am running a lot of DNA sequencing gels in my lab. One of the reagents we
use routinely is the TBE. We usually make a 10X stock and make dilution from
there. As many of you may already notice that 10XTBE will precipitate after
the stock is used/opened.  I am trying to get some chemistry explanations
about this precipitation. Is it because of the solubility, pH or chemical
reactions with air?  Thank you.

Xiaoyang Liu
Facility Scientist
Macromolecular Characterization Facility
The University of Connecticut
184 Auditorium Road, U-149
Storrs, Connecticut 06269-3149
Tel: 860-486-5024
Fax: 860-486-5005
e-mail: x_liu at biotek.mcb.uconn.edu

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