SDS and multimeric protein question

Anton Tutter atutter at aim.salk.edu
Thu Aug 12 23:11:39 EST 1999


> hello to everyone-
> i am working with a multimeric protein (it dimerizes and trimerizes), and i'm
> going to run it on a denaturing gel containing SDS. does this cause the
> multimer to dissociate into its constituent monomers?
> thanks
> -jen

sometimes--there may also be disulfide bonds forming between the multimers
especially if your protein contains many cysteine residues.  put plenty of
DTT in the gel mix (up to 5%) to minimize that potential problem.

good luck!

_______________________________________
Antonin Tutter
Salk Institute for Biological Studies
RBIO-J
10010 N. Torrey Pines Rd.
La Jolla, CA  92037
email:  tutter at salk.edu
web:  http://www-biology.ucsd.edu/~atutter/



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