SDS and multimeric protein question
atutter at aim.salk.edu
Thu Aug 12 23:11:39 EST 1999
> hello to everyone-
> i am working with a multimeric protein (it dimerizes and trimerizes), and i'm
> going to run it on a denaturing gel containing SDS. does this cause the
> multimer to dissociate into its constituent monomers?
sometimes--there may also be disulfide bonds forming between the multimers
especially if your protein contains many cysteine residues. put plenty of
DTT in the gel mix (up to 5%) to minimize that potential problem.
Salk Institute for Biological Studies
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email: tutter at salk.edu
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