the problem on plasmid ligation and transformation

YATHIN95 yathin95 at aol.com
Fri Aug 13 06:41:20 EST 1999


My experience with PCR products is first to clone into T vector (refer
Invitrogen or promega Cat) and the release the product from this vector. This
makes sure of your cutting at both ends. Now subclone into your vector of
choice. Try it it works!

Rauf



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