Richard P. Grant
r_grant at see.sig.for.address
Fri Aug 13 07:54:13 EST 1999
In article <Pine.GSO.3.96.990809162338.7713A-100000 at alpha.qmw.ac.uk>,
D.Schnapp at qmw.ac.uk ("D.Schnapp") wrote:
>remember just boiling up the membranes in some water plus - I think- EDTA
>(not sure whether it was EDTA or why it was added).
Sounds about right. Boil in 1 mM EDTA, (you should smaell the sulphur
preservative coming out) discard, repeat, boil in 18.2 water, store at 4C
under 50% EtOH. Rinse well before use.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
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