ricardo.silva at CIGB.EDU.CU
Sun Aug 15 23:31:51 EST 1999
> I am running a lot of DNA sequencing gels in my lab. One of the reagents we
> use routinely is the TBE. We usually make a 10X stock and make dilution from
> there. As many of you may already notice that 10XTBE will precipitate after
> the stock is used/opened. I am trying to get some chemistry explanations
> about this precipitation. Is it because of the solubility, pH or chemical
> reactions with air? Thank you.
I once read in an old Biotechniques that filtering the TBE stock
through a 0.45 um filter solves the problem. The authors claimed
they could routinely store 50 X stocks!! There was also some
blurbing about dust particles being nucleation centers for TBE.
More information about the Methods