Stripping DIG-northerns

Jonathan Howarth jrh2 at st-andrews.ac.uk
Wed Aug 18 03:41:10 EST 1999


Hello,

I wondered if anyone could help me. I am trying to probe northerns with
DIG-labelled DNA probes several times but have not yet got any
hybridisation after the first stripping. I am using the boiling 0.1% SDS
method.

Does anyone know if this is common? According to Boehringers user manual
this should be OK. 

Also if anyone knows whether I would get better results using in vitro
transcribed RNA probes could let me know.

Thanks in advance,
Jonathan Howarth

NB. Please reply to jonathan.howarth at bbsrc.ac.uk rather than the address
from which this was sent. 





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