Really random is DNA breakage
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Aug 18 08:57:45 EST 1999
In article <7pdtv6$i8m$1 at niobium.hgmp.mrc.ac.uk>, Mr. M.J. Lush
<mlush at hgmp.mrc.ac.uk> writes
>This is why
>I'm wondering if there are any known bias in cleavage by DNaseI or
I haven't noticed any reports of bias. I would just go ahead and try
both. With an 8kb target you shouldn't need too many colonies to get a
truly representative library. Have look at some of the gene shuffling
papers (July Nature Biotechnology springs to mind) for DNAse conditions.
They tend to chop up targets to 20-40bp so with a bit of playing you
should be able to get the size range you want.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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