RNA pol-expressing plasmids?

Chris Kafer ckafer at iastate.edu
Wed Aug 18 10:16:46 EST 1999


Article Title 
Single-step purification of T7 RNA polymerase with a 6-histidine Tag 

Article Abstract 
Single-step purification of T7 RNA polymerase with a 6-histidine tag 

Article Authors 
Ellinger, T.; Ehricht, R. 

Citation 
(Benchmarks, BioTechniques 24:718-720, May 1998) 

Topic Keywords 
T7 RNA polymerase; histidine tag; His tag 




On Wed, 18 Aug 1999 09:13:25 +0200, "Dr. Fernando Rodriguez-Pascual"
<frodrigu at mail.uni-mainz.de> wrote:

>Hi users,
>
>In my lab we are using a Taq pol-expression plasmid in order to get a
>cheap enzyme prep for our PCR experiments. Because we also do lot in
>vitro RNA transcription reactions now I wonder if there is anybody who
>has RNA polymerase (T3, T7 and SP6)-expressing plasmids and wants to
>share them with us.
>
>Cheers
>
>Fernando
>-- 
>#############################################
>Dr. Fernando Rodríguez Pascual
>Department of Pharmacology
>Johannes Gutenberg University
>Obere Zahlbacher Str. 67
>D-55101 Mainz, Germany
>Tf. +49 (6131) 17 68 36 or 17 32 45
>Fax. +49 (6131) 17 66 11
>email: frodrigu at mail.uni-mainz.de
>#############################################




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