Request for a 6x gel loading buffer receipt

Dave A. Vadnais dvadnais at uoguelph.ca
Wed Aug 18 11:49:12 EST 1999


Hello Martin.
I use the following recipe with good results.
50% glycerol
0.4% Bromophenol blue
10 mM Sodium Phosphate (pH 7.0)
It is a 10x loading buffer
Cheers
Dave

Martin Chan wrote:

> I have tried making a 6x gel loading buffer using the following receipt:
>
> 0.25% bromophenol blue
> 0.25% xylene cyanol FF
> 30% glycerol
> But the resulting solution adheres to the outside of pipette tips and is
> therefore difficult to pipette.
> Can anyone provide me another receipt?
> Thanks in advance.




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