Reducing agents for protein chemistry

Achim Recktenwald, PhD ARecktenwald at StressGen.com
Wed Aug 18 14:49:00 EST 1999


So far, I haven't received any satisfying answers to my post.

Achim



Jun Zhang <junz at sfu.ca> wrote in message
news:199908181553.IAA00098 at fraser.sfu.ca...

> I would like to know the answer too. Thanks.
>
> >
> > Hi everybody,
> >
> > For some time already, I am looking for a paper / book / webpage / or
any
> > other source that contains a list of redox potentials for commonly used
> > reducing agents, like:
> >
> > beta-mercaptoethanol (BME), dithiothreitol (DTT), glutathione, cysteine,
> > Tris(2-carboxyethyl)phosphine hydrochloride (TCEP.HCl) and so on. There
must
> > be others around, as well.
> >
> >
> > In many references you can find that DTT is about 10fold better than
BME.
> > But I found the wording always so similar that I assume the authors are
just
> > copying from each other.
> > Does anybody know of a more exact reference, possibly even including
more
> > compounds?
> >
> > We have to produce proteins for in vivo experiments and cannot use BME
or
> > DTT in final preparations, because of their toxicity. In addition, I
would
> > be interested in their relative strength, i.e., their redox potential.
> >
> >
> > Any answer would be appreciated If possible, send me a copy of your
answer
> > also by email
> >
> > Thanks,
> >
> > Achim
> >
> > ARecktenwald at StressGen.com
> >
> >
> >
> >
> >
>





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