fast DNA from mammalian cells

Nick ihanvan at imvs.sa.gov.au
Mon Aug 16 01:59:16 EST 1999


You can lyse the cells quite easily in 0.5% NP-40.
I currently use this in an RT-PCR protocol with .5% NP40 in superscript
buffers and it busts them open a treat.
P.S. don't forget to mix well!.

Nick

Mircea Podar wrote in message ...
>Hello!
>
>Does anyone have a fast protocol to get DNA for PCR from a small number of
>cells in culture? I presume just throwing the cells in the PCR mix would
>work (I've done it with yeast and coli), but I would like a cleaner way,
>and that way the mini DNA sample can be used repeatedly over several days.
>
>
>Thanks,
>
>Mircea Podar
>





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