stabilization of disulfide bonds by oxidation: HOW?

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Fri Aug 20 07:11:18 EST 1999


Hi all,
We have immobilized a cystein containing protein on thiopropyl sepharose

beads where is bound via a single disulfide bond. The protein can be
quantitatively recovered from the beads by treatment with DTT. Now, we
want to make this bond resistant to DTT, and have thought about
oxidation of the bond to a sulfonate. What would be the best method to
do that? The reaction should be simple to perform, quantitative, and
with minimal side reactions with other amino acids including methionine.

Any ideas?

Frank




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