Request for a 6x gel loading buffer receipt

Nacer Bellaloui nibellaloui at ucdavis.edu
Fri Aug 20 12:35:49 EST 1999



> Hello Martin,

I have always used your formula.  It sticks a little to the inside of my tips,
but I just ignore it. The gels come out fine.  However, if this is a problem
for you I suggest you buy low retention pipette tips.  I once saw them
demonstrated and they really seem to work well.


>
> Martin Chan wrote:
>
> > I have tried making a 6x gel loading buffer using the following receipt:
> >
> > 0.25% bromophenol blue
> > 0.25% xylene cyanol FF
> > 30% glycerol
> > But the resulting solution adheres to the outside of pipette tips and is
> > therefore difficult to pipette.
> > Can anyone provide me another receipt?
> > Thanks in advance.




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