Peptide solubility and aa-composition

Pal Szecsi d189456 at
Sat Aug 21 02:26:18 EST 1999

Download the free program from
It is a Win (only) small program that tells you about the problems with
synthetic peptides.
It calculate the MW, pI & hydrophobicity and estimate its solubility. It
also tells you when sequences are difficult to syntheze.
You can also order peptides directly from it.

Pal Szecsi M.D.Ph.D.
Dept of Clinical Chemistry
Roskilde County Hospital
DK-4000 Roskilde

Troels Wind wrote:

> Dear netters,
> I intend to order a synthetic peptide to be used in both protein-binding
> assays and in cell cultures. The sequence is a mostly hydrophobic
> heptamer (A, T, W, L and G residues) flanked by two cysteines that
> should form a constrained loop by disulfide-bridging.
> My worry is whether the hydrofobicity of the peptide will cause
> solubility-problems.
> Does any of you have experience with such peptides ? Do you know a 'rule
> of thumb' for the correlation between aa-composition and solubility in
> water or DMSO ?
> Any comments would be appreciated!
> Cheers,
> Troels
> --
> Troels Wind, Ph.D.
> University of Aarhus
> Dept. of Molecular and Structural Biology
> Gustav Wieds Vej 10C
> DK-8000 Aarhus C
> Denmark
> Phone +45 8942 5052 or 5078
> Fax +45 8612 3178
> E-mail wind at
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