? Immunizing Protocol ?

jongrak kittiworakarn jongrak at HOTMAIL.COM
Mon Aug 23 00:48:36 EST 1999

Dear Alls:

I have immunized the hen with an antigen for the IgY.  The antigen
has been SDS-PAGE purified. The target band was cut and mixed with the
adjuvant employed.
I immunized with Freund's Complete Adjuvant (FCA) on the day 0,
followed by Freund's Incomplte Adjuvant (FIA) on the day 14, 21 and 28.

However, I found that the antibody also cross react with other proteins.

The previuosly immunized hen did not be booste for the last two months.
I would like to try reimmunizing the hen with other types of adjuvant
or route of administration.

Since it was not recommended to re-immunized with FCA, what adjuvant I
should try?  What is the recommended  immunization schedule for the


-----------------------Jongrak  Kittiworakarn
Dept. of Biochem.
Fact. of Pharmacy
Mahidol Univ.
Bangkok 10400, Thailand.

More information about the Methods mailing list