supercoiled DNA

J. Martinez-Irujo jjmirujo at
Tue Aug 24 08:18:27 EST 1999

Hello all,

Does anyone know a method to isolate supercoiled plasmid DNA without
making use of a CsCl gradient? This DNA will be used as substrate in a
deoxyribonuclease assay (0,1 to 1 mg would be desirable). Thanks.

J.  Martinez-Irujo

Departamento de Bioquimica
Universidad de Navarra
Pamplona, Spain

e-mail:  jjmirujo at

More information about the Methods mailing list