PCR high GC long template

Souichi Ikeno ikeno at ac.shoyaku.ac.jp
Tue Aug 24 03:44:54 EST 1999


> Andrew Leung wrote:
>
> > Hi,
> >
> > Which Polymerase should I used to amplify GC> 70% , >2kb template?
> > It should be a high fidelity one, because I need to subclone the PCR
> > fragment. Would the addition of PCR enhancer generally reduce fidelity?
> >
> > Thank you.
> > Andrew
>

Hi  Andrew,

  You should use TaKaRa  LA  Taq (TaKaRa, Code No.RR002A).
We are successful in amplifying DNA fragment (up to 10-kb).

Souichi Ikeno




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