Absorbance of DNA at 260/280 nm

Richard P. Grant r_grant at see.sig.for.address
Wed Aug 25 07:00:48 EST 1999


Hi,

Heard someone say that if DNA was really really clean that if you run a
wavelength scan of it, instead of a relatively broad peak from ~230 ->
~300 nm that centres on 260 nm (OK, 259 nm for the pedants), you get a
peak at 260 nm and then a separate one at 280 nm.  Is this complete BS or
is there some truth in it?

R

-- 
Richard P. Grant MA DPhil         |         rgrant at cmtech.co.uk
home: www.avnet.co.uk/adastra     |         work: www.cmtech.co.uk
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