Absorbance of DNA at 260/280 nm

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Aug 25 08:06:57 EST 1999


Richard P. Grant <r_grant at see.sig.for.address> wrote:
> Heard someone say that if DNA was really really clean that if you run a
> wavelength scan of it, instead of a relatively broad peak from ~230 ->
> ~300 nm that centres on 260 nm (OK, 259 nm for the pedants), you get a
> peak at 260 nm and then a separate one at 280 nm.  Is this complete BS or
> is there some truth in it?

Sounds rather strange to me. What is supposed to cause the peak at 280 nm?
The bases all have their absorption peak around 260 nm. Just put some
ATP in the spec and watch.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



More information about the Methods mailing list