Absorbance of DNA at 260/280 nm

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Wed Aug 25 09:55:52 EST 1999


Never saw that! Not even a hint of such "twin peaks" with the cleanest preps
(CsCl)!



At 01:00 PM 8/25/99 +0100, Richard P. Grant wrote:
>Hi,
>
>Heard someone say that if DNA was really really clean that if you run a
>wavelength scan of it, instead of a relatively broad peak from ~230 ->
>~300 nm that centres on 260 nm (OK, 259 nm for the pedants), you get a
>peak at 260 nm and then a separate one at 280 nm.  Is this complete BS or
>is there some truth in it?
>
>R
>
>-- 
>Richard P. Grant MA DPhil         |         rgrant at cmtech.co.uk
>home: www.avnet.co.uk/adastra     |         work: www.cmtech.co.uk
>      What did you say your username was?  <clickety-click>   
>
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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