PCR Problems

Deborah Sims SimsD at agric.unp.ac.za
Thu Aug 26 06:03:09 EST 1999


I am an honours student having problems with my PCR reactions.  I keep
getting amplification in my control lane which is supposed to have water
in instead of DNA.  I have checked for contamination but all the
reagents appear to be clean! Is it possible that the primers are somehow
being amplified?  Any comments or advice would be greatly appreciated.

Thank You 
Debbie Sims

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