Achim Recktenwald, PhD ARecktenwald at
Thu Aug 26 12:44:34 EST 1999

Martin Offterdinger <a8803349.nospam at> wrote in message
news:37c524f5.342622 at
> What is the purpose in using iodoacetamide for protein isolation. (I
> found it in a protocol) Does it inhibit (thiol-) proteases? Could it
> also be used to neutralize DTT or BME?

Iodoacetamide is an alkylating reagent for cysteines.
Strange to use it during a protein purification. Normally it's used to
alkylate the cysteines of the final product, e.g.,  for protein chemical
procedures, like peptide mapping, etc.


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