Ian A. York iayork at
Thu Aug 26 12:54:30 EST 1999

In article <62fx3.944$2%3.6496215 at>,
Achim Recktenwald, PhD <ARecktenwald at> wrote:
>Martin Offterdinger <a8803349.nospam at> wrote in message
>news:37c524f5.342622 at
>> What is the purpose in using iodoacetamide for protein isolation. (I
>Iodoacetamide is an alkylating reagent for cysteines.
>Strange to use it during a protein purification. Normally it's used to

Iodoacetamide is (among many other things) a protease inhibitor,
irreversibly inhibiting (not surprisingly) cysteine proteases.  It's often
used in protein purification for exactly that reason.

    Ian York   (iayork at  <>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England

More information about the Methods mailing list