PCR Problems

Mustafa Bahadir Aktan aktan at purdue.edu
Thu Aug 26 13:00:13 EST 1999

recheck again everything, especially your enzyme...

Deborah Sims wrote:
> Hi
> I am an honours student having problems with my PCR reactions.  I keep
> getting amplification in my control lane which is supposed to have water
> in instead of DNA.  I have checked for contamination but all the
> reagents appear to be clean! Is it possible that the primers are somehow
> being amplified?  Any comments or advice would be greatly appreciated.
> Thank You
> Debbie Sims

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