how to amplify a GC-rich DNA by PCR

Don Walthers gene_jockey26 at yahoo.com
Fri Aug 27 16:02:50 EST 1999


i find that extended denaturing and annealing times (2 minutes) help. for my
primers 95C and 48C work for denaturning and annealing temps. also try %5
DMSO.

--
Don Walthers
Microbiology
University of Idaho
gene_jockey26 at yahoo.com





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