How can make a >4% agarose gel?

John R. McQuiston zje8 at cdc.gov
Mon Aug 30 09:26:11 EST 1999


I would use Metaphor agarose at 2.5 - 3.0%.  It'll resolve to 4 bp easily.

It boils over easily in a microwave so go slowly.

John




In article <37CA7D47.78AB3D1D at hotmail.com>, genetic_judge at hotmail.com says...
>
>I find that 2% agarose solution in 1x TAE is already quite viscous. Is
>it technically possible to prepare 4% or more agarose gel? If yes, then
>how to achieve that and how about the resolution of agarose gel when
>compared with that of polyacrylamide gel for size below 100bp?
>
>Sorry for so many questions.
>Thanks in advance.




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