DNA extraction from gel

Mike Cooley cooley at waksman.rutgers.edu
Mon Aug 30 16:42:49 EST 1999


Rick Bright wrote:

> GeneClean Spin does not use NaI at all.
>
> Anne Gillen wrote:
> >
> > > There are kits by Qiagen, Bio101 and probably by many other companies. These
> > > I know are based on gel solubilization by NaI. High salt concentration make
> > > DNA bound to silica gel (silica particles, silica membrane or whatever),
> > > then washing the resin with alcohol and elution with water. These methods
> > > work well as RNA/DNA extraction methods too if you substitute NaI with
> > > GuSCN.
> > >
> > > Cheers,
> > > Tomek
> >
> > Tomek,
> >
> > I know this may sound like nit picking, but I once had someone tell me in all
> > seriousness that you did not need NaI to use the gene clean on PCR fragments
> > (Sometimes I use the kit to clean up PCR, not bands in agarose) because it is
> > only needed to solubilize the agarose.  This is not so.  I looked up the
> > original paper and it said that NaI, or other chaotropic ions, are needed to
> > bind DNA to the silica.  Once the DNA is bound you no longer need the NaI to
> > maintain the bond.
> >
> > Anne

Since when? The bottles were labeled as NaI.


--
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Dr. Michael Cooley
Waksman Institute
Rutgers University
190 Frelinghuysen Rd.
Piscataway, NJ 08854

732-445-3802
FAX 732-445-5735
cooley at waksman.rutgers.edu




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