How can make a >4% agarose gel?
mb at laplace.csb.yale.edu
Tue Aug 31 08:07:05 EST 1999
I've successfully made agarose gels (using Nusieve 3:1) as high as 6%.
I've used this to see if inserts as small as 60bp are being digested.
It's a matter of not getting too much air on the sample during the
melting. I pre-dissolve the agarose powder on a stir plate for awhile
before heating. During microwave heating I never allow the sample to
boil without stopping and mixing. FMC has a great deal of info on this
More information about the Methods