perkin elmer vs light cycler

Dr. Duncan Clark Duncan at
Tue Aug 31 10:25:43 EST 1999

In article <7qgptv$8ee at>, Joan Marie Shields
<jshields at> writes
>Is the MgCl2 there for the polymerase

The polymerase, but an equimolar amount is titrated out by the dNTPs
i.e. 0.2mM each dNTP takes out 0.8mM MgCl2 leaving 0.7mM for the enzyme.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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