Why does high salt favour DNA strand hybriisation?
s.riceNOcySPAM at genesis.co.nz
Mon Aug 30 19:52:09 EST 1999
I have often wondered why higher NaCl concentrations favour
DNA strand hybridisation, e.g. in a Southern blot. I have
never seen anyone try to explain why. My guess is that Na+
ions could shield the -ve charges of the phosphate backbone,
which might otherwise tend to cause the 2 DNA strands to
repel each other.
Anyone out there have any ideas?
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