transfecting NG108-15 cells

Marco Domeniconi mdomeniconi at gc.cuny.edu
Thu Dec 9 15:41:28 EST 1999


I have transfected NG108-15 cells using  LipofectAMINE Plus and medium with
serum in 6 well plates.
I used the serum-free to form the lipid-DNA complexes but added serum to the
transfection medium. The efficiency I observed was only about 5% lower than
the serum-free protocol.
Hope it helps
Marco

Caroline Szymeczek-Seay wrote:

> Hi,
> Just checking to see if anyone has transfected NG108-15 cells...I've
> found only one reference in the literature for NG108-15 tranfection
> using Lipofectamine.  That's fine, but I'd prefer to try one of the
> newer reagents that doesn't require that the cells be transfected in
> serum-free media.  has anyone tried the quigen effectene reagent, or any
> other similar reagent?
>
> thanks for input...
>
> clss




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