using Taq in fill-in reactions

Jens Tornoe jt at neurosearch.dk
Sat Dec 11 16:17:36 EST 1999


> If you want to do blunt cloning, use Taq/Pfu mix with ALL 4 nucleotides.
>

When I do blunt clonings, I use Pfu exclusively. It works fine. What would
be the advantage of adding Taq?

Regards,

Jens


--
Jens Tornøe
NeuroSearch
Pederstrup, Denmark
http://www.neurosearch.com





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