using Taq in fill-in reactions

Jens Tornoe jt at
Sat Dec 11 16:17:36 EST 1999

> If you want to do blunt cloning, use Taq/Pfu mix with ALL 4 nucleotides.

When I do blunt clonings, I use Pfu exclusively. It works fine. What would
be the advantage of adding Taq?



Jens Tornøe
Pederstrup, Denmark

More information about the Methods mailing list