FACS: excluding dead cells?

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Dec 21 10:53:09 EST 1999


In article <Pine.A41.4.05.9912210910370.47780-100000 at node9.unix.Virginia.EDU>, Lionel  Berthoux <lb8x at node9.unix.Virginia.EDU> wrote:
>Hi,
>
>I am FACscanning cells using GFP + a PE-labeled Ab. I would like to
>exclude contaminating dead cells. Does anybody know of a reagent that
>would be useful in that context? (I think 7AAD is not compatible with the
>use of PE).
>

Propidium iodide is a plasma membrane impermeable nuclear dye. 
Dead cells will have all of their DNA stained, live cells won't uptake it.

Or simply light scattering signal will separate dead and living into two 
distinct groups. 

        - Dima





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