Methods December 1999 Archives by author

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Starting: Wed Dec 1 02:47:34 EST 1999
Ending: Thu Dec 30 15:15:51 EST 1999
Messages: 456

BAD expression in broth "", David Alexander
bistronic e. coli expression Slawomir Dabrowski 18-22
Generic DNA binding protein Achim Recktenwald, PhD
*** IMPORTANT: BIONET hardware upgrade and reorganization *** BIOSCI Administrator
isolation of DNA from plasma? Jason Affourtit
Statistics software Ashok Aiyar
Pay off Your Debts Alex
Methylataion Specific PCR Jennifer F Anders
trypsinize cell before making RNA? Ulf Andersson
ISH-formamide Jose Andrade
need help for handling 293 cell Ahmet Arman
need help for handling 293 cell Ahmet Arman
DNA Strider David Asch
Myotubes transfection protocol wanted Peter Ashby
Myotubes transfection protocol wanted Peter Ashby
Taq patent Dan Ballard
Sequnecing gel problems... Beat at hgmp.mrc.ac.uk
ISH-formamide Belscotia
Protein purification Bernard Murray, PhD
strider file format conversion? Bernard Murray, PhD
RT best enzyme? thermostable? Bernard Murray, PhD
Immunoprecipitation question... Bernard Murray, PhD
trypsinize cell before making RNA? Bernard Murray, PhD
T-vector? Bernard Murray, PhD
FACS: excluding dead cells? Lionel Berthoux
Looking for pics/sites for teaching Flourescence in Biology Bill_A_Nussbaumer at ms.bd.com
RT best enzyme? thermostable? BjornP
GST dimers Alex Blais
heterologous expression problem in E. coli Frederik Boernke
Yeast Two-hybrid Library Kent S. Boles
Sensitivity Kent S. Boles
New Mol Biol web site Mark Bond
western blotting -pvdf Mark Bond
2-h y b r i d: how many groups Mark Bond
PCR amplification problems could use some help Bonehammer
Disposal of EtBr Bonehammer
Western stripping Younes Bounhar
PCR amplification problems could use some help Gregor Bucher
PCR amplification problems could use some help Gregor Bucher
Coomassie staining of proteins on PVDF - The answer? Thorsten Burmester
Disposal of EtBr CSC
EST sequences: 5' or 3' end?? Alexandre Caetano
Helix Cafe-The Life Sciences Community Site Helix Cafe
Equilibrium Dialysis Help Please Martin Cann
determination of Km Enrique Castro
Cloning in pGEM-T Francesca Catalano
ligation Francesca Catalano
PCR amplification problems could use some help Francesca Catalano
visible calcium phosphate-DNA precipitate? Bruno Cenni
Mutation Software Robert Chadwick
Cutting phage DNA Zhonglin Chai
Cutting with MboII Martin Chan
rna isolation T V Naga Rama Chander
Detection of a hybrid DNA molecule Li Changbao
GST dimers ChenHA
Orthovanadate??? Steve Christov
minimum F1 ori coordinates for phagemid construction Dr. Duncan Clark
ligation problems Dr. Duncan Clark
d/ddNTP ratios for Cycle sequencing with Taq Dr. Duncan Clark
gene rearrangement Dr. Duncan Clark
Taq patent Dr. Duncan Clark
Taq patent Dr. Duncan Clark
Why doesn't my cloning work? Water? Dr. Duncan Clark
SYBR Green I & PCR inhibition? Dr. Duncan Clark
Open season on Taq Dr. Duncan Clark
Coomassie absorber? Dr. Duncan Clark
PCR amplification of the FULL 16S rDNA bacterial gene Dr. Duncan Clark
T7phageselect - hands on anyone ? Dr. Duncan Clark
PCR amplification of the FULL 16S rDNA bacterial gene Dr. Duncan Clark
Stratagene thermal cycler users Dr. Duncan Clark
Anything new about BioRad iCycler? Dr. Duncan Clark
transfection reagents - a second update Dr. Duncan Clark
ligation Jacquelyn Clark
viral bicistronic vectors Scott Craig
Differences between Detergents DOCTORHIM at aol.com
ligation Stephen Dahl
Immunoprecipitation question... Stephen Dahl
GST dimers Stephen Dahl
Taq patent Stephen Dahl
Taq patent Stephen Dahl
2-H y b r i d: How many groups?? Stephen Dahl
peptide antibody production Stephen Dahl
2-H y b r i d: How many groups?? Stephen Dahl
Help! Does anyone have a PCR Protocol for BIG Primers (Forward=20mer, Reverse=40mer...Tm is ~83C) Dandog
POST-DOCTORAL position, Tours, France Daniel.Marc
need help for handling 293 cell David L. Haviland, PhD
f1 gene II, supplier? Clinton Dilks
Liver cDNA GAL4 Yeast two hybrid library Dirk
transfecting NG108-15 cells Marco Domeniconi
Taq patent Rich Dudley
Disposal of EtBr E.Post
BAD expression in broth Roger Fachini
Transfection reagents Frank O. Fackelmayer
Help! Does anyone have a PCR Protocol for BIG Primers (Forward=20mer, Reverse=40mer...Tm is ~83C) Frank O. Fackelmayer
2-H y b r i d: How many groups?? Frank O. Fackelmayer
Coomassie absorber? Frank O. Fackelmayer
mung bean nuclease problem Frank O. Fackelmayer
Gel filtration vs gradient Frank O. Fackelmayer
Multiple bands of HAT-fusion product Frank O. Fackelmayer
nuclear DNA content in diploid cells ? Frank O. Fackelmayer
Myotubes transfection protocol wanted Frank O. Fackelmayer
transfection reagents - a second update Frank O. Fackelmayer
transfection reagents - a second update Frank O. Fackelmayer
transfection reagents - a second update Frank O. Fackelmayer
Myotubes transfection protocol wanted Frank O. Fackelmayer
Myotubes transfection protocol wanted Frank O. Fackelmayer
transfection reagents - a second update Frank O. Fackelmayer
transfection reagents - a second update Frank O. Fackelmayer
Help us create a methods database! Jeff Fairman
Magnetic Beads Jeff Fairman
Statistics software Jeff Fairman
Stratagene thermal cycler users Joyce Faler
Stratagene thermal cycler users Joyce Faler
Poly T seqencing John Fellers
GAL4 antibody desperately needed! Esbjorn Fiers
T-vector? S. Findley
f1 gene II, supplier? Andy Finn
PCR amplification problems could use some help Tim Fitzmaurice
Disposal of EtBr Tim Fitzmaurice
ISH-formamide George Flentke
ief gels Will Fuller
Diff Gene Exp: Best Method ? James Funderburgh
Generic DNA binding protein Gary
micrococcal nuclease (the use of) Dr. Peter Gegenheimer
mung bean nuclease problem Dr. Peter Gegenheimer
micrococcal nuclease Yoram Gerchman
micrococcal nuclease Yoram Gerchman
micrococcal nuclease Yoram Gerchman
micrococcal nuclease (the use of) Yoram Gerchman
Arabinose like promotor Yoram Gerchman
B lymphocyte transfection and expression Dennis Gor
AFLP newsgroup? Silke Gorissen
(NONE) Silke Gorissen
Open season on Taq Richard P. Grant
Open season on Taq Richard P. Grant
Open season on Taq Richard P. Grant
ANNC:ABRF MICROARRAY USER SURVEY Chandi Griffin
colony PCR Xin Guo
DuoLux Xin Guo
? The mechanism of protein precipitation by heavy metal ? H.V.Taylor
Genotyping by PCR Chen HA
colony PCR Chen HA
Precipitate in Tris solution due to acetic acid??? Andre Hamel
Good place to buy laboratory workbench? Robert Hartley
Sensitivity Robert Hartley
trypsinize cell before making RNA? Robert Hartley
Stratagene thermal cycler users Robert Hartley
adsorbing/conjugating proteins to polycarbonate membranes Robert Hartley
color font? Robert Hartley
Stratagene thermal cycler users Robert Hartley
Submit Your Site NOW!! Robert Hartley
Statistics software Robert Hartley
trypsinize cell before making RNA? David L. Haviland
transfection reagents - a second update David L. Haviland
transfection reagents - a second update David L. Haviland
Re, DuoLux Zheng-Hui He
Sheep,Goat Genomic Libraries Steven Hecht
TOPO cloning experiences? J. Hendriks
Antibody to PAR ? Michael Herron
Enzyme linked Streptavidin Ina Hinners
nuclear DNA content in diploid cells ? Carsten Hohoff
d/ddNTP ratios for Cycle sequencing with Taq Nick van Holst
FACS: excluding dead cells? Nick van Holst
Magnetic Beads Louis Hom
Yeast and temperature David Humair
Storage of archival 0.2ml PCR tubes? Olav Hungnes
Open season on Taq Roland Hübner
Open season on Taq Roland Hübner
EF-BOS expression plasmid "stuffer" mystery Wendy Ingram
CLONTECH RevTET system JAC
Wheat Genomic library VASANTHARAJAN JANAKIRAMAN
Removal of PCR inhibitors JKCarson
veterinary journal impact factor ITABAJARA DA SILVA VAZ JUNIOR
Sequnecing gel problems... Jernej Jakse
f1 gene II, supplier? Bart Janssen
T-vector? Paul Jasper
sequencing services R. Jayakumar
PCR amplification problems could use some help R. Jayakumar
Disposal of EtBr R. Jayakumar
sequencing services Ma Sheila M. De Jesus
ligation Ying Jiang
SYBR Green I & PCR inhibition? David R. Johnson
Get Paid to review MolBio enzymes, reagents, antibodies etc. Ted Jones
Orthovanadate??? Jose C. Juarez
d/ddNTP ratios for Cycle sequencing with Taq KIM
Longmire's solution KIM
protocol for PCR product purification with silica resin? Chris Kafer
colony PCR Chris Kafer
anti-thioG Abs Dr. Eugene S. Kandel
Immunoprecipitation of CAT reporter protein, which antibody? Aletta Karsies
Magnetic Beads Narendra Kaushik
Magnetic Beads Narendra Kaushik
Poly T seqencing Jeffrey Keen
Yeast medium Byung-Hoon Kim
Differences between Detergents Byung-Hoon Kim
Differences between Detergents Byung-Hoon Kim
poly dI-dC versus salmon sperm DNA Byung-Hoon Kim
expression in mammalian cells Sangwon Kim
Passive Lysis Buffer and Westerns Rob Kirkpatrick
expression in mammalian cells Rob Kirkpatrick
Strange "Christmas Tree" Lanes on Western Rob Kirkpatrick
Stable transfected cell lines by co-transfection, Svend Kjaer
T7phageselect - hands on anyone ? Svend Kjaer
Electroporator Unit Dima Klenchin
GST dimers Dima Klenchin
Differences between Detergents Dima Klenchin
FACS: excluding dead cells? Dima Klenchin
? The mechanism of protein precipitation by heavy metal ? Dima Klenchin
Myotubes transfection protocol wanted Dima Klenchin
? The mechanism of protein precipitation by heavy metal ? Dima Klenchin
Equilibrium Dialysis Help Please Dima Klenchin
Myotubes transfection protocol wanted Dima Klenchin
T-vector? Rory Koenen
ligation Rory Koenen
BIOLOGICAL FOOD Rory Koenen
heterologous expression problem in E. coli Rory Koenen
Britton-Robinson Marianne Koivulehto
colony PCR Paul Kowalski
determination of Km Jacob Koziarz
Differences between Detergents Cornelius Krasel
Differences between Detergents Cornelius Krasel
Differences between Detergents Cornelius Krasel
32P-dNTP in PCR Cornelius Krasel
Removal of Guanidine-HCl from protein sample???? Kresten
Removal of Guanidine-HCl from protein sample???? Kresten
DsRED Brian K. Kristensen
gene rearrangement Terry Lau
T-vector? Terry Lau
BOOM and NASBA Paula Lavery
Expression library screening Alan Lee
Western blotting -PVDF Sa Mi Lee
Magnetic Beads Klaus Lehnert
Removal of Guanidine-HCl from protein sample???? Andrew Leung
Retroviral gene transfer Andrew Leung
Multiple bands of HAT-fusion product Andrew Leung
ER visual localisation vector Andrew Leung
viral bicistronic vectors Victor Levenson
Question about AHC media for yeast Liang Liang
Western blotting -PVDF Andrew Liddell
2-H y b r i d: How many groups?? Martin Linan
2-H y b r i d: How many groups?? Martin Linan
Help: Site-directed mutagenesis Chris H Lindley
Western blotting -PVDF Chris H Lindley
0.2 ml PCR tubes - cold Chris H Lindley
Mutations: sftwre for finding and aligning? John Little
Info on Kretach? Muhammad A. Lodhi
Fluorescent labeling of DNA and RNA Muhammad A. Lodhi
Taq patent R. John Lye
Coomassie absorber? R. John Lye
unable to precipitate DNA from CONCERT miniprep kit Rafael Maldonado
Source of P1 vir Rafael Maldonado
2-H y b r i d: How many groups?? Rafael Maldonado
Coomassie absorber? Rafael Maldonado
unable to precipitate DNA from CONCERT miniprep kit Marcos
Free Christmas screensaver advert Seasons Marketing
PCR with whole bacteria Damien Marsic
Removal of Guanidine-HCl from protein sample???? Alejandro Martin
Removal of Guanidine-HCl from protein sample???? Alejandro Martin
Bradford protein determination; calibration curve J. Martinez-Irujo
viral bicistronic vectors Luis Miguel Martins
using Taq in fill-in reactions Luis Miguel Martins
trypsinize cell before making RNA? Bernhard Mayr
Nuclear ion concentration - EMSA Bernhard Mayr
Western stripping Neil McKenna
Coomassie absorber? Neil McKenna
FLAG tag detection on Western analysis Neil McKenna
adsorbing/conjugating proteins to polycarbonate membranes Michael Andres McMurray
Electroporator Unit John R. McQuiston
Help: Site-directed mutagenesis John R. McQuiston
ligation John R. McQuiston
Orthovanadate??? Neal Robert Melvin
Immunoprecipitation question... Neal Robert Melvin
Question about expression library screening... Neal Robert Melvin
strider file format conversion? Myc
Southern blots Frauke Naumann
polish 5'overhang/cloning Frauke Naumann
mung bean nuclease problem Frauke Naumann
mung bean nuclease problem Frauke Naumann
Disposal of EtBr Mario Nenno
Magnetic Beads William of Occam
Magnetic Beads William of Occam
Western stripping Martin Offterdinger
sequencing services Dr Yoshitaka Ohashi
poly dI-dC versus salmon sperm DNA Eric Chace Olivares
Sequnecing gel problems... Eric Chace Olivares
DNA Strider Orac
FLAG tag detection on Western analysis Nick Owen
how to find an antibody on the internet: The Antibody Resource Page
Gel filtration vs gradient Dave Parcej
HELP: PCR and formaldehyde Parent, Eric
stratagene thermal cycler users Parent, Eric
Taq patent Pathos
Genotyping by PCR Patrick.Mcmanamny at med.monash.edu.au
ief gels Sean Patterson
immunopricipitation question Peter
Plant ATPase Anti-body Alfredo Lopez De leon Ph.D.
Equilibrium Dialysis Help Please John Philo
0.2 ml PCR tubes - cold Michael Prigge
DNA Strider Kiley R. Prilliman
Gelatin in 10X Yeast PCR Buffer Cyril Privenzentzev
immunopricipitation question Kun Qian
Disposal of EtBr Jean-Paul RAFFIN
GST dimers Ed Ralph
visible calcium phosphate-DNA precipitate? Colin Rasmussen
Magnetic Beads Colin Rasmussen
GST dimers Colin Rasmussen
poly dI-dC versus salmon sperm DNA Sybille Rex
ligation Susanne Rohrer
PCR amplification problems could use some help Susanne Rohrer
RNA-DNA partition in phenol extraction Susanne Rohrer
Question about primer extension assay? Susanne Rohrer
Pay Off Your Debts Ronny
Cutting phage DNA Hiranya S. Roychowdhury
ligation Hiranya S. Roychowdhury
Protein purification Hiranya S. Roychowdhury
Coomassie absorber? Hiranya S. Roychowdhury
Coomassie absorber? Hiranya S. Roychowdhury
Taq patent George Rutherford
PCR amplification problems could use some help George Rutherford
Strange "Christmas Tree" Lanes on Western George Rutherford
Help: Site-directed mutagenesis Rux
Help: Site-directed mutagenesis Rux
rna isolation Neil Saunders
Myotubes transfection protocol wanted Wolfgang Schechinger
Anything new about BioRad iCycler? Wolfgang Schechinger
Myotubes transfection protocol wanted Wolfgang Schechinger
Cutting phage DNA Joerg Schenk
Antibody against P2y2 Hermann Schillers
ligation problems Kai Schmengler
PCR amplification problems could use some help Jarmo Schrader
fosmid cloning Erich Seemueller
fosmid cloning Erich Seemueller
gene rearrangement P.J. Shaw
need help for handling 293 cell A.F. Simpson
Transfection reagents A.F. Simpson
Magnetic Beads A.F. Simpson
PCR amplification problems could use some help A.F. Simpson
AFLP newsgroup? Dan Skinner
Question about primer extension assay? Alan Smith
f1 gene II, supplier? Harold E Smith
Longmire's solution Lee Smith
Immunoprecipitation question... Stephen Snowdy
Magnetic Beads Stephen Snowdy
Magnetic Beads Stephen Snowdy
Graduate Opportunities in Plant Physiology/Molecular Biology Martin Spalding
transfecting NG108-15 cells Caroline Szymeczek-Seay
ligation Nick Theodorakis
Immunoprecipitation question... Nick Theodorakis
trypsinize cell before making RNA? Nick Theodorakis
Taq patent Nick Theodorakis
Differences between Detergents Nick Theodorakis
nuclear DNA content in diploid cells ? Nick Theodorakis
PCR amplification problems could use some help Anne Thomas
EF-BOS expression plasmid "stuffer" mystery Gordon Thomas
peptide antibody production Stephanie Toering
ligation Jens Tornoe
PCR amplification problems could use some help Jens Tornoe
Immunoprecipitation question... Jens Tornoe
Sensitivity Jens Tornoe
PCR amplification problems could use some help Jens Tornoe
colony PCR Jens Tornoe
using Taq in fill-in reactions Jens Tornoe
colony PCR Jens Tornoe
colony PCR Jens Tornoe
PCR with whole bacteria Jens Tornoe
Transfection reagents Michael Tuvim
RT best enzyme? thermostable? Michael Tuvim
Transfection reagents Michael Tuvim
transfection reagents - an update Michael Tuvim
Removal of Guanidine-HCl from protein sample???? Tyson
Western blotting -PVDF Tyson
Coomassie absorber? Tyson
CaPO4 Chris Upton
CaPO4 Chris Upton
mung bean nuclease problem Kevin Urbanic
Help,I need a good kit to make a human cDNA bank !!!! Nasrin Vasseur
Coomassie absorber? Henk Veldman
Coomassie absorber? Henk Veldman
fluorescent tag for primer Perumal Vijayan
Removal of Guanidine-HCl from protein sample???? Arnoud van Vliet
colony PCR Arnoud van Vliet
Good place to buy laboratory workbench? WSchick at AOL.COM
32P-dNTP in PCR Shiao Wang
0.2 ml PCR tubes - cold Shiao Wang
anything new about biorad icycler Weining, Song
O-acetyl-DL-homoserine source? Kathryn Weiss
Sensitivity Chong Wai Yin( Zhang Weixian)
colony PCR Chong Wai Yin( Zhang Weixian)
Enzyme linked Streptavidin Chong Wai Yin( Zhang Weixian)
calculate Tm Chong Wai Yin( Zhang Weixian)
Happy new year! Chong Wai Yin( Zhang Weixian)
PCR amplification problems could use some help Scott C Winkel
HELP: PCR and formaldehyde Jan-Henner Wurmbach
Help: Site-directed mutagenesis Jan-Henner Wurmbach
32P-dNTP in PCR Minsoo Yoon
Immunoprecipitation question... Ian A. York
Immunoprecipitation question... Ian A. York
Western blotting -PVDF Ian A. York
transfection reagents - a second update Ian A. York
Protein purification Mike Zharkovsky
His-tagged (E)GFP fusion protein mammalian expression vector? Mike Ziegner
information about subscription to bio net aarman at CAREGROUP.HARVARD.EDU
PCR with whole bacteria abcd
DNA Strider aniya
Statistics software armstrong800 at my-deja.com
trypsinize cell before making RNA? baffulo at my-deja.com
visible calcium phosphate-DNA precipitate? bcttcb at my-deja.com
Kit for proteolytic activity? capcoon
GAL4 antibody desperately needed! cathy.slack at ic.ac.uk
Taq polymerase trivia tom chappell
using Taq in fill-in reactions tom chappell
Yeast Two-hybrid Library chenauxp at biology.queensu.ca
PCR ckf
dilution of ddATP alex dobrovic
Caylase substitute dpicton
BOOM and NASBA elader at ambion.com.nospam
Stratagene thermal cycler users elader at ambion.com.nospam
HELP: PCR and formaldehyde harvey
Dyes? if not I'll die harvey
Differences between Detergents hzhen at freeuk
color font? jaakko.hyvonen at utu.fi
Enzyme linked Streptavidin kang.liu at worldnet.att.net
f1 gene II, supplier? karlster
? The mechanism of protein precipitation by heavy metal ? jongrak kittiworakarn
Good place to buy laboratory workbench? paul lacy
Why doesn't my cloning work? Water? ladasky at my-deja.com
Why doesn't my cloning work? Water? ladasky at my-deja.com
AFLP newsgroup? ladasky at my-deja.com
Anything new about BioRad iCycler? lors
Coomassie absorber? mayday at newsguy.com
bistronic e. coli expression mb
Magnetic Beads medimag at my-deja.com
Magnetic Beads medimag at my-deja.com
calculate Tm nvahsen at hot.hgmp.mrc.ac.uk
isolation of DNA from plasma? nvahsen at hot.hgmp.mrc.ac.uk
Detection limit from dye-JOE j. ouwehands
Taq patent pathos
(none) joshua prabhakar
protocol for PCR product purification with silica resin? - An inexpensive alternative to glassmilk.doc (0/1) fred quimby
PCR amplification of the FULL 16S rDNA bacterial gene rcaspi at ucsd.edu
PCR amplification of the FULL 16S rDNA bacterial gene rcaspi at ucsd.edu
Immunoprecipitation question... rm
colony PCR rogier
Palmitoylation inhibitor rousso at gaiberg.wi.mit.edu
ligation sarlab
Enzyme linked Streptavidin sballal at wockhardtin.com
ligation problems schmeng at uni-muenster.de
retroviral vectors schneema at yahoo.com
Submit Your Site NOW!! srchzznngnz at juno.com
0.2 ml PCR tubes - cold tfitzwater at gilead.com
Strange "Christmas Tree" Lanes on Western tfitzwater at gilead.com
Request Yersinia Christmas humor post bradley turner
How to combine GeneMap and dbEST data? unknown
How to combine GeneMap and dbEST data? unknown
SDS-PAGE question (skewed protein bands) zach
protocol for PCR product purification with silica resin? Liang zhang

Last message date: Thu Dec 30 15:15:51 EST 1999
Archived on: Sun Mar 6 22:07:17 EST 2005

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