Methods December 1999 Archives by thread

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Starting: Wed Dec 1 02:47:34 EST 1999
Ending: Thu Dec 30 15:15:51 EST 1999
Messages: 456

Taq polymerase trivia tom chappell
Removal of PCR inhibitors JKCarson
Cutting phage DNA Joerg Schenk
- Cutting phage DNA Zhonglin Chai
Removal of Guanidine-HCl from protein sample???? Andrew Leung
- Removal of Guanidine-HCl from protein sample???? Alejandro Martin
  - Removal of Guanidine-HCl from protein sample???? Kresten
    - Removal of Guanidine-HCl from protein sample???? Alejandro Martin
- Removal of Guanidine-HCl from protein sample???? Tyson
- Removal of Guanidine-HCl from protein sample???? Kresten
- Removal of Guanidine-HCl from protein sample???? Arnoud van Vliet
Cutting with MboII Martin Chan
HELP: PCR and formaldehyde Parent, Eric
- HELP: PCR and formaldehyde Jan-Henner Wurmbach
- HELP: PCR and formaldehyde harvey
Detection of a hybrid DNA molecule Li Changbao
Yeast and temperature David Humair
BOOM and NASBA Paula Lavery
- BOOM and NASBA elader at ambion.com.nospam
Passive Lysis Buffer and Westerns Rob Kirkpatrick
Cutting phage DNA Hiranya S. Roychowdhury
Caylase substitute dpicton
Generic DNA binding protein Gary
- Generic DNA binding protein Achim Recktenwald, PhD
Info on Kretach? Muhammad A. Lodhi
Fluorescent labeling of DNA and RNA Muhammad A. Lodhi
TOPO cloning experiences? J. Hendriks
CaPO4 Chris Upton
- CaPO4 Chris Upton
Help: Site-directed mutagenesis Rux
- Help: Site-directed mutagenesis Jan-Henner Wurmbach
  - Help: Site-directed mutagenesis Chris H Lindley
  - Help: Site-directed mutagenesis John R. McQuiston
    - Help: Site-directed mutagenesis Rux
BAD expression in broth "", David Alexander
- BAD expression in broth Roger Fachini
unable to precipitate DNA from CONCERT miniprep kit Marcos
- unable to precipitate DNA from CONCERT miniprep kit Rafael Maldonado
immunopricipitation question Kun Qian
- immunopricipitation question Peter
Electroporator Unit Dima Klenchin
- Electroporator Unit John R. McQuiston
ligation problems schmeng at uni-muenster.de
viral bicistronic vectors Luis Miguel Martins
- viral bicistronic vectors Scott Craig
- viral bicistronic vectors Victor Levenson
Sheep,Goat Genomic Libraries Steven Hecht
Coomassie staining of proteins on PVDF - The answer? Thorsten Burmester
Yeast Two-hybrid Library chenauxp at biology.queensu.ca
- Yeast Two-hybrid Library Kent S. Boles
Yeast medium Byung-Hoon Kim
His-tagged (E)GFP fusion protein mammalian expression vector? Mike Ziegner
Western stripping Neil McKenna
- Western stripping Martin Offterdinger
- Western stripping Younes Bounhar
Good place to buy laboratory workbench? paul lacy
- Good place to buy laboratory workbench? Robert Hartley
dilution of ddATP alex dobrovic
- need help for handling 293 cell Ahmet Arman
  - need help for handling 293 cell David L. Haviland, PhD
    - need help for handling 293 cell Ahmet Arman
  - need help for handling 293 cell A.F. Simpson
Question about AHC media for yeast Liang Liang
ligation Ying Jiang
- ligation Stephen Dahl
- ligation John R. McQuiston
  - ligation Nick Theodorakis
  - ligation Jens Tornoe
- ligation Rory Koenen
- ligation Jacquelyn Clark
protocol for PCR product purification with silica resin? Liang zhang
- protocol for PCR product purification with silica resin? - An inexpensive alternative to glassmilk.doc (0/1) fred quimby
  - protocol for PCR product purification with silica resin? Chris Kafer
Good place to buy laboratory workbench? WSchick at AOL.COM
Protein purification Mike Zharkovsky
minimum F1 ori coordinates for phagemid construction Dr. Duncan Clark
ligation problems Kai Schmengler
- ligation problems Dr. Duncan Clark
DsRED Brian K. Kristensen
sequencing services Dr Yoshitaka Ohashi
- sequencing services R. Jayakumar
- sequencing services Ma Sheila M. De Jesus
ligation Hiranya S. Roychowdhury
- ligation Francesca Catalano
  - ligation Susanne Rohrer
Protein purification Hiranya S. Roychowdhury
- Protein purification Bernard Murray, PhD
Dyes? if not I'll die harvey
Diff Gene Exp: Best Method ? James Funderburgh
determination of Km Jacob Koziarz
- determination of Km Enrique Castro
information about subscription to bio net aarman at CAREGROUP.HARVARD.EDU
Transfection reagents Michael Tuvim
- Transfection reagents Frank O. Fackelmayer
  - Transfection reagents Michael Tuvim
  - Transfection reagents A.F. Simpson
strider file format conversion? Myc
- strider file format conversion? Bernard Murray, PhD
RT best enzyme? thermostable? Michael Tuvim
- RT best enzyme? thermostable? Bernard Murray, PhD
- RT best enzyme? thermostable? BjornP
retroviral vectors schneema at yahoo.com
PCR amplification problems could use some help Scott C Winkel
- PCR amplification problems could use some help Jens Tornoe
  - PCR amplification problems could use some help Anne Thomas
  - PCR amplification problems could use some help Susanne Rohrer
    - PCR amplification problems could use some help Jens Tornoe
    - PCR amplification problems could use some help A.F. Simpson
    - PCR amplification problems could use some help Tim Fitzmaurice
    - PCR amplification problems could use some help Gregor Bucher
- PCR amplification problems could use some help Francesca Catalano
  - PCR amplification problems could use some help Jarmo Schrader
- PCR amplification problems could use some help Bonehammer
veterinary journal impact factor ITABAJARA DA SILVA VAZ JUNIOR
Orthovanadate??? Neal Robert Melvin
- Orthovanadate??? Steve Christov
- Orthovanadate??? Jose C. Juarez
Immunoprecipitation question... Neal Robert Melvin
- Immunoprecipitation question... Ian A. York
  - Immunoprecipitation question... Jens Tornoe
    - Immunoprecipitation question... Ian A. York
    - Immunoprecipitation question... Stephen Dahl
  - Immunoprecipitation question... Nick Theodorakis
- Immunoprecipitation question... Bernard Murray, PhD
  - Immunoprecipitation question... rm
- Immunoprecipitation question... Stephen Snowdy
Free Christmas screensaver advert Seasons Marketing
Sensitivity Chong Wai Yin( Zhang Weixian)
- Sensitivity Jens Tornoe
  - Sensitivity Robert Hartley
    - Sensitivity Kent S. Boles
Stable transfected cell lines by co-transfection, Svend Kjaer
trypsinize cell before making RNA? baffulo at my-deja.com
- trypsinize cell before making RNA? Ulf Andersson
- trypsinize cell before making RNA? Nick Theodorakis
- trypsinize cell before making RNA? Bernhard Mayr
- trypsinize cell before making RNA? David L. Haviland
- trypsinize cell before making RNA? Robert Hartley
- trypsinize cell before making RNA? Bernard Murray, PhD
Mutations: sftwre for finding and aligning? John Little
Source of P1 vir Rafael Maldonado
fluorescent tag for primer Perumal Vijayan
d/ddNTP ratios for Cycle sequencing with Taq Nick van Holst
- d/ddNTP ratios for Cycle sequencing with Taq KIM
  - d/ddNTP ratios for Cycle sequencing with Taq Dr. Duncan Clark
Mutation Software Robert Chadwick
visible calcium phosphate-DNA precipitate? bcttcb at my-deja.com
- visible calcium phosphate-DNA precipitate? Bruno Cenni
- visible calcium phosphate-DNA precipitate? Colin Rasmussen
Plant ATPase Anti-body Alfredo Lopez De leon Ph.D.
Cloning in pGEM-T Francesca Catalano
gene rearrangement Terry Lau
- gene rearrangement Dr. Duncan Clark
- gene rearrangement P.J. Shaw
Retroviral gene transfer Andrew Leung
Wheat Genomic library VASANTHARAJAN JANAKIRAMAN
ief gels Will Fuller
- ief gels Sean Patterson
ligation sarlab
Magnetic Beads Stephen Snowdy
- Magnetic Beads Klaus Lehnert
  - Magnetic Beads medimag at my-deja.com
  - Magnetic Beads medimag at my-deja.com
    - Magnetic Beads A.F. Simpson
  - Magnetic Beads William of Occam
- Magnetic Beads Jeff Fairman
- Magnetic Beads Louis Hom
- Magnetic Beads Stephen Snowdy
  - Magnetic Beads Colin Rasmussen
Help us create a methods database! Jeff Fairman
Graduate Opportunities in Plant Physiology/Molecular Biology Martin Spalding
how to find an antibody on the internet: The Antibody Resource Page
T-vector? Terry Lau
- T-vector? Rory Koenen
- T-vector? Paul Jasper
  - T-vector? Bernard Murray, PhD
- T-vector? S. Findley
Antibody against P2y2 Hermann Schillers
(none) joshua prabhakar
bistronic e. coli expression mb
- bistronic e. coli expression Slawomir Dabrowski 18-22
GST dimers Alex Blais
- GST dimers Colin Rasmussen
- GST dimers Stephen Dahl
- GST dimers Dima Klenchin
- GST dimers Ed Ralph
- GST dimers ChenHA
Taq patent Dr. Duncan Clark
- Taq patent Nick Theodorakis
  - Taq patent pathos
- Taq patent R. John Lye
- Taq patent Stephen Dahl
  - Taq patent Dr. Duncan Clark
    - Taq patent Dan Ballard
    - Taq patent George Rutherford
  - Taq patent Rich Dudley
    - Taq patent Pathos
  - Taq patent Stephen Dahl
*** IMPORTANT: BIONET hardware upgrade and reorganization *** BIOSCI Administrator
DNA Strider David Asch
- DNA Strider Orac
  - DNA Strider Kiley R. Prilliman
- DNA Strider aniya
CLONTECH RevTET system JAC
Longmire's solution Lee Smith
- Longmire's solution KIM
Open season on Taq Richard P. Grant
- Open season on Taq Roland Hübner
  - Open season on Taq Richard P. Grant
    - Open season on Taq Roland Hübner
    - Open season on Taq Dr. Duncan Clark
    - Open season on Taq Richard P. Grant
BIOLOGICAL FOOD Rory Koenen
Magnetic Beads Narendra Kaushik
- Magnetic Beads William of Occam
heterologous expression problem in E. coli Rory Koenen
using Taq in fill-in reactions Luis Miguel Martins
- using Taq in fill-in reactions tom chappell
  - using Taq in fill-in reactions Jens Tornoe
PCR ckf
RNA-DNA partition in phenol extraction Susanne Rohrer
transfecting NG108-15 cells Caroline Szymeczek-Seay
- transfecting NG108-15 cells Marco Domeniconi
heterologous expression problem in E. coli Frederik Boernke
EST sequences: 5' or 3' end?? Alexandre Caetano
O-acetyl-DL-homoserine source? Kathryn Weiss
Methylataion Specific PCR Jennifer F Anders
Why doesn't my cloning work? Water? ladasky at my-deja.com
- Why doesn't my cloning work? Water? Dr. Duncan Clark
  - Why doesn't my cloning work? Water? ladasky at my-deja.com
SYBR Green I & PCR inhibition? David R. Johnson
- SYBR Green I & PCR inhibition? Dr. Duncan Clark
transfection reagents - an update Michael Tuvim
Help! Does anyone have a PCR Protocol for BIG Primers (Forward=20mer, Reverse=40mer...Tm is ~83C) Dandog
- Help! Does anyone have a PCR Protocol for BIG Primers (Forward=20mer, Reverse=40mer...Tm is ~83C) Frank O. Fackelmayer
Magnetic Beads Narendra Kaushik
peptide antibody production Stephanie Toering
- peptide antibody production Stephen Dahl
2-H y b r i d: How many groups?? Martin Linan
- 2-H y b r i d: How many groups?? Stephen Dahl
- 2-H y b r i d: How many groups?? Martin Linan
  - 2-H y b r i d: How many groups?? Stephen Dahl
  - 2-H y b r i d: How many groups?? Frank O. Fackelmayer
    - 2-H y b r i d: How many groups?? Rafael Maldonado
colony PCR Chong Wai Yin( Zhang Weixian)
- colony PCR Arnoud van Vliet
- colony PCR Jens Tornoe
  - colony PCR Paul Kowalski
    - colony PCR Jens Tornoe
    - colony PCR Chen HA
    - colony PCR Jens Tornoe
- colony PCR Chris Kafer
  - colony PCR Xin Guo
- colony PCR rogier
B lymphocyte transfection and expression Dennis Gor
Immunoprecipitation of CAT reporter protein, which antibody? Aletta Karsies
Detection limit from dye-JOE j. ouwehands
expression in mammalian cells Sangwon Kim
- expression in mammalian cells Rob Kirkpatrick
Differences between Detergents Byung-Hoon Kim
- Differences between Detergents Cornelius Krasel
  - Differences between Detergents Byung-Hoon Kim
    - Differences between Detergents Cornelius Krasel
- Differences between Detergents Dima Klenchin
- Differences between Detergents hzhen at freeuk
mung bean nuclease problem Kevin Urbanic
- mung bean nuclease problem Dr. Peter Gegenheimer
Differences between Detergents DOCTORHIM at aol.com
- Differences between Detergents Nick Theodorakis
  - Differences between Detergents Cornelius Krasel
Southern blots Frauke Naumann
polish 5'overhang/cloning Frauke Naumann
mung bean nuclease problem Frauke Naumann
- mung bean nuclease problem Frank O. Fackelmayer
  - mung bean nuclease problem Frauke Naumann
ISH-formamide Jose Andrade
- ISH-formamide Belscotia
  - ISH-formamide George Flentke
Coomassie absorber? mayday at newsguy.com
- Coomassie absorber? Frank O. Fackelmayer
  - Coomassie absorber? Dr. Duncan Clark
    - Coomassie absorber? Rafael Maldonado
- Coomassie absorber? Henk Veldman
- Coomassie absorber? Tyson
  - Coomassie absorber? Neil McKenna
- Coomassie absorber? R. John Lye
PCR amplification problems could use some help R. Jayakumar
- PCR amplification problems could use some help George Rutherford
  - PCR amplification problems could use some help Gregor Bucher
micrococcal nuclease Yoram Gerchman
micrococcal nuclease Yoram Gerchman
micrococcal nuclease Yoram Gerchman
Genotyping by PCR Patrick.Mcmanamny at med.monash.edu.au
- Genotyping by PCR Chen HA
Expression library screening Alan Lee
Western blotting -PVDF Sa Mi Lee
- Western blotting -PVDF Tyson
- Western blotting -PVDF Ian A. York
- Western blotting -PVDF Chris H Lindley
- Western blotting -PVDF Andrew Liddell
Get Paid to review MolBio enzymes, reagents, antibodies etc. Ted Jones
Strange "Christmas Tree" Lanes on Western Rob Kirkpatrick
- Strange "Christmas Tree" Lanes on Western George Rutherford
Question about expression library screening... Neal Robert Melvin
Gelatin in 10X Yeast PCR Buffer Cyril Privenzentzev
f1 gene II, supplier? Bart Janssen
- f1 gene II, supplier? karlster
  - f1 gene II, supplier? Andy Finn
  - f1 gene II, supplier? Harold E Smith
- f1 gene II, supplier? Clinton Dilks
ANNC:ABRF MICROARRAY USER SURVEY Chandi Griffin
micrococcal nuclease (the use of) Yoram Gerchman
- micrococcal nuclease (the use of) Dr. Peter Gegenheimer
Kit for proteolytic activity? capcoon
Coomassie absorber? Hiranya S. Roychowdhury
- Coomassie absorber? Henk Veldman
Poly T seqencing John Fellers
- Poly T seqencing Jeffrey Keen
anti-thioG Abs Dr. Eugene S. Kandel
SDS-PAGE question (skewed protein bands) zach
Nuclear ion concentration - EMSA Bernhard Mayr
32P-dNTP in PCR Shiao Wang
- 32P-dNTP in PCR Cornelius Krasel
- 32P-dNTP in PCR Minsoo Yoon
0.2 ml PCR tubes - cold Shiao Wang
- 0.2 ml PCR tubes - cold Chris H Lindley
- 0.2 ml PCR tubes - cold Michael Prigge
Gel filtration vs gradient Frank O. Fackelmayer
- Gel filtration vs gradient Dave Parcej
Help,I need a good kit to make a human cDNA bank !!!! Nasrin Vasseur
Britton-Robinson Marianne Koivulehto
Coomassie absorber? Hiranya S. Roychowdhury
Pay off Your Debts Alex
POST-DOCTORAL position, Tours, France Daniel.Marc
Disposal of EtBr Mario Nenno
- Disposal of EtBr R. Jayakumar
- Disposal of EtBr Tim Fitzmaurice
  - Disposal of EtBr CSC
- Disposal of EtBr Bonehammer
- Disposal of EtBr E.Post
- Disposal of EtBr Jean-Paul RAFFIN
GAL4 antibody desperately needed! cathy.slack at ic.ac.uk
- GAL4 antibody desperately needed! Esbjorn Fiers
Multiple bands of HAT-fusion product Andrew Leung
- Multiple bands of HAT-fusion product Frank O. Fackelmayer
0.2 ml PCR tubes - cold tfitzwater at gilead.com
Storage of archival 0.2ml PCR tubes? Olav Hungnes
Strange "Christmas Tree" Lanes on Western tfitzwater at gilead.com
Enzyme linked Streptavidin Chong Wai Yin( Zhang Weixian)
- Enzyme linked Streptavidin Ina Hinners
- Enzyme linked Streptavidin kang.liu at worldnet.att.net
  - Enzyme linked Streptavidin sballal at wockhardtin.com
Precipitate in Tris solution due to acetic acid??? Andre Hamel
FLAG tag detection on Western analysis Nick Owen
- FLAG tag detection on Western analysis Neil McKenna
rna isolation T V Naga Rama Chander
- rna isolation Neil Saunders
Helix Cafe-The Life Sciences Community Site Helix Cafe
Equilibrium Dialysis Help Please Martin Cann
- Equilibrium Dialysis Help Please John Philo
  - Equilibrium Dialysis Help Please Dima Klenchin
PCR amplification of the FULL 16S rDNA bacterial gene rcaspi at ucsd.edu
- PCR amplification of the FULL 16S rDNA bacterial gene Dr. Duncan Clark
  - PCR amplification of the FULL 16S rDNA bacterial gene rcaspi at ucsd.edu
    - PCR amplification of the FULL 16S rDNA bacterial gene Dr. Duncan Clark
poly dI-dC versus salmon sperm DNA Sybille Rex
- poly dI-dC versus salmon sperm DNA Eric Chace Olivares
  - poly dI-dC versus salmon sperm DNA Byung-Hoon Kim
nuclear DNA content in diploid cells ? Carsten Hohoff
- nuclear DNA content in diploid cells ? Nick Theodorakis
- nuclear DNA content in diploid cells ? Frank O. Fackelmayer
Bradford protein determination; calibration curve J. Martinez-Irujo
T7phageselect - hands on anyone ? Svend Kjaer
- T7phageselect - hands on anyone ? Dr. Duncan Clark
Sequnecing gel problems... Jernej Jakse
- Sequnecing gel problems... Eric Chace Olivares
- Sequnecing gel problems... Beat at hgmp.mrc.ac.uk
Question about primer extension assay? Alan Smith
- Question about primer extension assay? Susanne Rohrer
isolation of DNA from plasma? Jason Affourtit
- isolation of DNA from plasma? nvahsen at hot.hgmp.mrc.ac.uk
Request Yersinia Christmas humor post bradley turner
Stratagene thermal cycler users Joyce Faler
- Stratagene thermal cycler users elader at ambion.com.nospam
  - Stratagene thermal cycler users Robert Hartley
    - Stratagene thermal cycler users Dr. Duncan Clark
- DuoLux Xin Guo
- Stratagene thermal cycler users Joyce Faler
  - Stratagene thermal cycler users Robert Hartley
fosmid cloning Erich Seemueller
calculate Tm Chong Wai Yin( Zhang Weixian)
- calculate Tm nvahsen at hot.hgmp.mrc.ac.uk
stratagene thermal cycler users Parent, Eric
FACS: excluding dead cells? Lionel Berthoux
- FACS: excluding dead cells? Dima Klenchin
FACS: excluding dead cells? Nick van Holst
? The mechanism of protein precipitation by heavy metal ? jongrak kittiworakarn
- ? The mechanism of protein precipitation by heavy metal ? Dima Klenchin
  - ? The mechanism of protein precipitation by heavy metal ? H.V.Taylor
    - ? The mechanism of protein precipitation by heavy metal ? Dima Klenchin
Anything new about BioRad iCycler? lors
- Anything new about BioRad iCycler? Dr. Duncan Clark
- Anything new about BioRad iCycler? Wolfgang Schechinger
Myotubes transfection protocol wanted Wolfgang Schechinger
- Myotubes transfection protocol wanted Peter Ashby
  - Myotubes transfection protocol wanted Frank O. Fackelmayer
    - Myotubes transfection protocol wanted Peter Ashby
    - Myotubes transfection protocol wanted Wolfgang Schechinger
    - Myotubes transfection protocol wanted Frank O. Fackelmayer
    - Myotubes transfection protocol wanted Frank O. Fackelmayer
    - Myotubes transfection protocol wanted Dima Klenchin
- Myotubes transfection protocol wanted Dima Klenchin
transfection reagents - a second update Frank O. Fackelmayer
- transfection reagents - a second update David L. Haviland
  - transfection reagents - a second update Frank O. Fackelmayer
- transfection reagents - a second update David L. Haviland
  - transfection reagents - a second update Frank O. Fackelmayer
- transfection reagents - a second update Ian A. York
  - transfection reagents - a second update Dr. Duncan Clark
    - transfection reagents - a second update Frank O. Fackelmayer
  - transfection reagents - a second update Frank O. Fackelmayer
AFLP newsgroup? Dan Skinner
- AFLP newsgroup? ladasky at my-deja.com
EF-BOS expression plasmid "stuffer" mystery Gordon Thomas
anything new about biorad icycler Weining, Song
AFLP newsgroup? Silke Gorissen
(NONE) Silke Gorissen
New Mol Biol web site Mark Bond
western blotting -pvdf Mark Bond
2-h y b r i d: how many groups Mark Bond
Palmitoylation inhibitor rousso at gaiberg.wi.mit.edu
How to combine GeneMap and dbEST data? unknown
- How to combine GeneMap and dbEST data? unknown
EF-BOS expression plasmid "stuffer" mystery Wendy Ingram
PCR with whole bacteria Damien Marsic
- PCR with whole bacteria Jens Tornoe
- PCR with whole bacteria abcd
Liver cDNA GAL4 Yeast two hybrid library Dirk
ER visual localisation vector Andrew Leung
Arabinose like promotor Yoram Gerchman
Pay Off Your Debts Ronny
Antibody to PAR ? Michael Herron
Happy new year! Chong Wai Yin( Zhang Weixian)
Re, DuoLux Zheng-Hui He
adsorbing/conjugating proteins to polycarbonate membranes Michael Andres McMurray
- adsorbing/conjugating proteins to polycarbonate membranes Robert Hartley
color font? jaakko.hyvonen at utu.fi
- color font? Robert Hartley
Looking for pics/sites for teaching Flourescence in Biology Bill_A_Nussbaumer at ms.bd.com
Submit Your Site NOW!! srchzznngnz at juno.com
- Submit Your Site NOW!! Robert Hartley
fosmid cloning Erich Seemueller
Statistics software armstrong800 at my-deja.com
- Statistics software Ashok Aiyar
- Statistics software Jeff Fairman
- Statistics software Robert Hartley

Last message date: Thu Dec 30 15:15:51 EST 1999
Archived on: Sun Mar 6 22:07:17 EST 2005

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