cDNA quantification

Christoph Kirsch kirsch at mpiz-koeln.mpg.de
Tue Feb 2 08:23:20 EST 1999


I would like to quantify first strand cDNA relative to other cDNAs
synthesised in parallel reaktions. I could of course add some P32 but
like to get around radioactivity. Did anyone try to use fluorecently
labeled primer for cDNA synthesis? Subsequent removal of primer should
allow quantification of even minute amounts relative to a second, third,
... cDNA synthesis. Does Reverse transciptase e.g. Superscript accept
5´-fluorecently labeled primer?




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