separation of biotin labelled IgG from unlabelled IgG

Dan Pashniak dpashnia at
Tue Feb 2 15:32:02 EST 1999

Hi there . . . 

I am looking for a method of separating hapten-labeled IgG, specifically, biotin labeled, 
from unlabeled IgG. I am considering using an affinity column bound with anti-biotin. 

It's simple to remove the unbound biotin from the reaction matrix . . . just use a good old 
PD-10 (G25) column (Pharmacia). But, what about the unlabeled IgG. Size exclusion certainly won't work; biotin is only ~250 Da. 

Would and affinity column work? 

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