help for a promoter

ipg at ipg at
Wed Feb 3 12:52:33 EST 1999

i have cloned a 5'flanking region of a gene and i have tried its
promoter function with a reporter gene assay.
the region is TATA less and without a recognizable CAAT box.
the results are very poor. the construct have a very low CAT activity.
How can i be sure that this sequence is a promoter?

Thank you
Rita Garuti
University of Modena

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