RACE PCR

Xavier Stien StienX at missouri.edu
Wed Feb 3 17:56:33 EST 1999


I have used the kit, my primers were a 23-mer GSP of 72°C with AP1 and a
20-mer GSP of 58°C with AP1 also. In both cases it worked fine.

Xavier Stien, stienx at showme.missouri.edu

Beat wrote in message <36b6c6ac.0 at pfaff.ethz.ch>...
>I am currently using Clonetech's RACE PCR kit.
>They recommend unsing primers with Tm>70C.
>Has anybody used this kit succesfully?
>What was the Tm of your genespecific primers (calculated by which method?)
>
>Thanks
>Beat
>
>Beat Reidy
>
>beat.reidy at ipw.agrl.ethz.ch





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