desalting/concentrating low MW proteins

ardibat at wanadoo.fr ardibat at wanadoo.fr
Wed Feb 3 15:25:49 EST 1999


In article <798aqf$lqu$1 at enyo.uwa.edu.au>,
  Eugene Hone <eugeneh at cyllene.uwa.edu.au> wrote:
> Hi,
>
> Does anyone know of/have a method for concentrating and desalting a solution
> containing a 4kDa protein?
>
> Cheers
>

One solution for your problem (the problem is that a 4kD protein is too small
for dialyse) is to use a G25 resin.
Just put the resin in a tube with a very small hole on the bottom, pour your
protein solution and give a very short centrifuge tour to collect the
concentrated protein.
For desalting... I don't know...

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