help for a promoter

Edgar Valencia edgar at CIFN.UNAM.MX
Wed Feb 3 15:19:17 EST 1999


Hi Rita

what about changing the reporter?
what about looking for the messaanger of the CAT gene under your
"promoter" or look for the original messanger (from the real gene)?
what about a gel retardation assay to see if something sticks to your
fragment? You could use several polimerases in these assays. Of course
this one will  tell it can be recognized, but isn't telling you is an
active promoter
I was also thinking about a primer extension assay, which could tell you
where the gene starts to be transcribed and look in the neighborhoods.
and finally, what about mutate your sequence and look for the messanger?

Just some thoughts
Edg




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