High background in FISH with TSA

Stefan Burde burde at lanl.gov
Thu Feb 4 10:38:27 EST 1999


Enrique,

If your mRNA is fairly abundant, you can probably get away with directly
labeled fluorescent probes.  They will give less signal per probe
molecule, but will also have much lower background.  If you need some
signal amplification, and endogenous biotin is likely to be present,
then digoxigenin should indeed work better.
Hope this helps,
Stefan

Stefan Burde
Los Alamos National Laboratory
Los Alamos, NM, EE.UU.
burde at lanl.gov



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