desalting/concentrating low MW proteins
Sebastian.Bunka at vu-wien.ac.at
Thu Feb 4 07:00:31 EST 1999
Eugene Hone <eugeneh at cyllene.uwa.edu.au> wrote:
> Does anyone know of/have a method for concentrating and desalting a solution
> containing a 4kDa protein?
Have a look in the sigma catalog - techware. They have
a product called Biodialyser using ultrafiltration mem-
branes. They offer membranes with 500 and 1000 Da nomi-
nal MW linit. Might work - never tried.
Just dialyse against ammonium acetate buffer, and then
lyophilize your protein solution. The NH4OAc is
volatile and can be mostly removed by lyophilization
(depending on the amino acid composition !! up to 99 or
more percent can be removed - ref. Handbook of HPLC for
the separation of AA, peptides and proteins, Vol II ed.
Hancock, CRC Press 1984). So you only concentrate the
protein but not the salt. Just dissolve in appropriate
amount of desired buffer.
Sebastian Bunka, Dr. med. vet
Inst. Med. Chemistry, Vet. University Vienna
Ph. +43-1-250 77 ext. 4208, Fax: ext. 4290
e-mail: Sebastian.Bunka at vu-wien.ac.at
More information about the Methods