if two estriction sites too close

Wendy Ingram w.ingram at cmcb.REMOVE-THIS-BIT.uq.edu.au
Fri Feb 5 03:42:08 EST 1999


I suggest you have a look at the appendix of the NEB catelogue (no
affiliations). They have a great section called "cleavage close to the end
of DNA fragments" which should help you out. The number of 'extra' bases
required next to the recognition sequence for cleavage at a high rate
differs from enzyme to enzyme, with some being more picky than others. If
one enzyme needs more of an overhang than the other, then cutting with the
fussy enzyme first can be adventagous.

Good Luck,

Wendy Ingram


In article <79e41a$369$1 at justice.csc.cuhk.edu.hk>,
s970854 at mailserv.cuhk.edu.hk (Andrew Leung) wrote:

> Hi all,
> 
>         Would there be possible to cut two restriction sites which are of 4 
> base pair apart (Not taking into account the restriction recognition 
> sequence)?
> 
> Andrew

-- 
Wendy Ingram (PhD Student)
Centre for Molecular and Cellular Biology
University of Queensland
Brisbane, Australia



More information about the Methods mailing list