if two estriction sites too close

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Feb 5 03:54:40 EST 1999

In article <79e41a$369$1 at justice.csc.cuhk.edu.hk>, Andrew Leung
<s970854 at mailserv.cuhk.edu.hk> writes
>Hi all,
>        Would there be possible to cut two restriction sites which are of 4 
>base pair apart (Not taking into account the restriction recognition 

It will be totally dependant upon which enzymes you are using. Check out
the back of the NEB catalogue as it gives some info on the minimum
requirements some RE's have beyond the recognition site.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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