cDNA synthesis

Terry Tcklau at hotmail.com
Sat Feb 6 06:02:10 EST 1999


Hello,

Recently, I would like to construct a cDNA library. After inserts were
ligated into plasmid, it would be transformed into E.coli. However, the
transformation efficiency is low. Then, all the clones were proceeded
mini-prep and analysed by gel electrophoresis. From the gel, it can be
seen that only few clones have inserts and the size is about 1k.( but
the size of cDNA is ranged from 500 to 7000 base pair.) Can anyone tell
me what is my problem.
Thank you in advance.

Terry




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